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Updated: Sep 8, 2026
13:48
Reverse Genetics Mediated Recovery of Infectious Murine Norovirus
Published on: June 24, 2012
5'-Terminal m-7G(5')ppp(5')G-m-p in vivo: identification in reovirus genome RNA
Abstract:
Methylated reovirus mRNA was synthesized in vitro in the presence of S-adenosyl-L-[methyl-3H]-methionine. Viral genome double-stranded RNA that was uniformly labeled with 32-P was isolated from purified virions. The RNAs were mixed and their 5'-terminal structures compared by electrophoretic and chromatographic analyses after enzymatic digestion. Both the mRNA and the corresponding strand in the genome RNA contain m-7G(5')ppp(5')G-m-pCp, indicating that infected cells synthesize viral RNA with blocked, methylated 5' termini.
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