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Neovascularization during venous thrombosis organization: a preliminary study
T W Wakefield1, M J Linn, P K Henke
1Jobst Vascular Surgery Laboratory, Section of Vascular Surgery, Department of Surgery, Division of Pulmonary and Critical Care Medicine, Ann Arbor, Michigan, USA.
Journal of Vascular Surgery
|November 5, 1999
Summary
Interleukin-8 (IL-8) promotes thrombus organization by enhancing neovascularization and leukocyte infiltration in venous thromboembolism. This study demonstrates IL-8
Area of Science:
- Vascular biology
- Thrombosis research
- Inflammation and immunology
Background:
- Venous thromboembolism (VTE) can lead to variable thrombus organization and recanalization rates.
- The chemokine interleukin-8 (IL-8) is present in thrombi long after initiation, suggesting a role in organization.
- Thrombus organization is hypothesized to involve neovascularization and leukocyte influx.
Purpose of the Study:
- To investigate the role of IL-8 in thrombus organization following VTE.
- To determine if IL-8 administration promotes neovascularization and leukocyte infiltration within thrombi.
Main Methods:
- Rats with induced inferior vena cava thrombosis received IL-8 or serum albumin.
- Thrombus neovascularization was assessed using colloidal carbon perfusion and fluorescein isothiocyanate dextran.
- Cellular infiltration (neutrophils, monocytes) and endothelial markers were analyzed via histology.
Main Results:
- IL-8 treatment significantly increased neutrophil and monocyte infiltration into thrombi.
- Neovascularization, confirmed by colloidal carbon and dextran fluorescence, was significantly enhanced by IL-8 at day 4.
- IL-8-treated thrombi showed increased spindle-shaped cells, indicative of fibroblasts and endothelial cells.
Conclusions:
- Thrombus organization is a process involving neovascularization.
- Interleukin-8 (IL-8) significantly augments thrombus organization by promoting neovascularization and leukocyte recruitment.