Related Experiment Video

Updated: Aug 14, 2026

Real-time Live Imaging of T-cell Signaling Complex Formation
10:31

Real-time Live Imaging of T-cell Signaling Complex Formation

Published on: June 23, 2013

Intracellular expression of CD61 precedes surface expression

G Käfer1, A Willer, W Ludwig

  • 1III. Medizinische Klinik Mannheim, University of Heidelberg, Wiesbadenerstrasse 7-11, D-68305 Mannheim, Germany.

Annals of Hematology
|November 7, 1999
PubMed

Insights

This study identifies acute megakaryoblastic leukemia by detecting intracytoplasmic CD61 expression in myeloid blasts. This finding aids in differentiating early acute myeloid leukemia subtypes with unclear morphology.

Area of Science:

  • Hematology
  • Oncology
  • Immunophenotyping

Background:

  • Acute myeloid leukemia (AML) classification relies on morphology, immunophenotype, and cytogenetics.
  • Differentiating AML subtypes, particularly those with undifferentiated morphology like FAB-M0 and early FAB-M7, can be challenging.
  • Megakaryoblastic differentiation is typically identified by cell surface markers.

Observation:

  • A case of acute myeloid leukemia presented with agranular blasts negative for myeloperoxidase.
  • Flow cytometry revealed myeloid markers (HLA-DR, CD7, CD13, CD33, CD34) and intracytoplasmic CD61 expression.
  • Cytogenetic analysis identified a deletion on chromosome 7: del(7)(q21-22).

Findings:

  • Intracytoplasmic CD61 expression was confirmed via flow cytometry after cell permeabilization.
  • This suggests that cytoplasmic CD61 expression may precede cell surface expression.
  • The myeloid origin was confirmed by a panel of myeloid-associated antigens.

Implications:

  • Detecting intracytoplasmic CD61 is crucial for diagnosing early acute megakaryoblastic leukemia (FAB-M7).
  • This method can help distinguish FAB-M7 from FAB-M0 in cases with undifferentiated morphology and negative cytochemistry.
  • Investigating cytoplasmic CD61 expression offers a valuable diagnostic tool in challenging AML cases.

More Related Videos

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking09:01

Integrate Imaging Flow Cytometry and Transcriptomic Profiling to Evaluate Altered Endocytic CD1d Trafficking

Published on: October 29, 2018

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses11:06

Preparation of Bead-supported Lipid Bilayers to Study the Particulate Output of T Cell Immune Synapses

Published on: April 1, 2022

Related Concept Videos

Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...