Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNA probe specific to Streptococcus sobrinus.

H Ida1, T Igarashi, A Yamamoto

  • 1Department of Pediatric Dentistry, Showa University School of Dentistry, Tokyo, Japan.

Oral Microbiology and Immunology
|November 7, 1999
PubMed
Summary

A specific DNA probe, SSB-3, was developed to identify Streptococcus sobrinus. This probe effectively detects and differentiates S. sobrinus from other oral streptococci, aiding in bacterial identification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cloning of a cDNA encoding the Tcp-1 (t complex polypeptide 1) homologue of Arabidopsis thaliana.

Gene·1992
Same author

The primary structure of porcine aminoacylase 1 deduced from cDNA sequence.

Journal of biochemistry·1992
Same author

Crystal structure of papain-succinyl-Gln-Val-Val-Ala-Ala-p-nitroanilide complex at 1.7-A resolution: noncovalent binding mode of a common sequence of endogenous thiol protease inhibitors.

Biochemistry·1992
Same author

A point mutation of low-density-lipoprotein receptor causing rapid degradation of the receptor.

European journal of biochemistry·1992
Same author

[Concept of hyperlipidemia and the clinical problems].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·1992
Same author

Initial clinical experience with a single pass VDDR pacing system.

Pacing and clinical electrophysiology : PACE·1992

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Streptococcus sobrinus is an oral bacterium implicated in dental caries.
  • Accurate identification of S. sobrinus is crucial for epidemiological studies and clinical diagnostics.

Purpose of the Study:

  • To develop and evaluate a specific DNA probe for the detection and identification of Streptococcus sobrinus.

Main Methods:

  • Polymerase chain reaction (PCR) was used to amplify three DNA fragments (SSB-1, -2, and -3) from the dextranase gene (dex) of S. sobrinus.
  • These fragments were used as DNA probes to assess hybridization specificity and sensitivity with DNA from various oral streptococcal species.

Main Results:

  • Probes SSB-1 and SSB-2 showed cross-reactivity with Streptococcus downei.

Related Experiment Videos

  • Probe SSB-3 demonstrated high specificity for S. sobrinus, detecting as little as 5 ng of chromosomal DNA or DNA from 1 x 10(5) cells.
  • SSB-3 successfully differentiated clinical isolates of S. sobrinus from Streptococcus mutans.

    • Conclusions:

    • The DNA probe SSB-3 is highly specific and sensitive for the detection and identification of Streptococcus sobrinus.
    • SSB-3 serves as an effective tool for distinguishing S. sobrinus from other related oral streptococci.