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Related Experiment Videos

Identification of 4 different alternatively spliced HLA-A transcripts.

L Norgaard1, L Fugger, H O Madsen

  • 1Department of Clinical Immunology, University Hospital of Copenhagen (Rigshospitalet), Denmark. larsnorgaard@hotmail.com

Tissue Antigens
|November 7, 1999
PubMed
Summary

Researchers discovered alternative splicing in HLA-A transcripts, generating smaller products. This phenomenon, observed in cancer patients, may help cancer cells evade immune surveillance.

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Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Cancer Research

Background:

  • Developing sequencing-based typing (PCR-SBT) for the HLA-A locus.
  • Observed unexpected lower molecular weight bands during PCR.
  • These bands were present despite PCR optimization.

Purpose of the Study:

  • Investigate the nature of the smaller PCR products.
  • Determine the cause of the additional bands observed in HLA-A typing.
  • Elucidate the phenomenon of alternative splicing in HLA-A transcripts.

Main Methods:

  • Polymerase Chain Reaction (PCR)-Sequencing Based Typing (SBT)
  • Cloning and sequencing of PCR products
  • Analysis of HLA-A transcripts

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Main Results:

  • Identified alternative splicing of HLA-A transcripts as the cause of smaller products.
  • Detected transcripts lacking exon 3, exons 2 and 3, exons 3 and 4, and exons 2-4.
  • Observed this phenomenon in samples from colorectal cancer and myeloma patients, and a colon tumor cell line.

Conclusions:

  • Alternative splicing of HLA-A transcripts occurs to a small extent in most cells.
  • The biological significance of these alternatively spliced HLA-A variants is currently unknown.
  • These alternatively spliced transcripts may contribute to immune evasion in cancer.