Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vaults and telomerase share a common subunit, TEP1.

V A Kickhoefer1, A G Stephen, L Harrington

  • 1Department of Biological Chemistry, UCLA School of Medicine and Jonsson Comprehensive Cancer Center, Los Angeles, California 90095, USA. vkick@mednet.ucla.edu

The Journal of Biological Chemistry
|November 7, 1999
PubMed
Summary

Mammalian vaults, large RNP complexes, contain TEP1, also known as the telomerase-associated protein 1. Despite TEP1

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Listeria Meningitis, one of your five a day? A case report of Listeria Monocytogenes Meningitis in a fit and well 62-year-old woman.

Acute medicine·2023
Same author

Attitudes of women with gestational diabetes toward diet and exercise: a qualitative study.

The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·2023
Same author

Developing priorities for quality improvement in acute medicine using a modified Delphi method A consensus process hosted by the Society for Acute Medicine Quality Improvement Committee (SAM-QI).

Acute medicine·2022
Same author

A Qualitative Evaluation of a Simulation Training Initiative for Registrars Working in Acute Medicine.

Acute medicine·2022
Same author

AeDES: a next-generation monitoring and forecasting system for environmental suitability of Aedes-borne disease transmission.

Scientific reports·2020
Same author

Opioid analgesia and the somatosensory memory of neonatal surgical injury in the adult rat.

British journal of anaesthesia·2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Vaults are large, enigmatic cytoplasmic ribonucleoprotein complexes.
  • Mammalian vaults are composed of high molecular mass proteins, including a 240-kDa component.
  • The function of vaults remains largely undetermined.

Purpose of the Study:

  • To identify the 240-kDa vault protein.
  • To investigate the relationship between vaults and telomerase.
  • To elucidate the role of TEP1 in vault structure and function.

Main Methods:

  • Partial cDNA sequencing to identify the 240-kDa vault protein.
  • Yeast three-hybrid assays to study RNA-protein interactions.
  • Biochemical assays to assess telomerase activity in vaults.

Related Experiment Videos

Main Results:

  • The 240-kDa vault protein was identified as TEP1 (telomerase-associated protein 1), the mammalian homolog of Tetrahymena p80.
  • TEP1 interacts with mammalian telomerase RNA and hTERT.
  • Vault particles contain TEP1 but exhibit no detectable telomerase activity.
  • Human vault RNAs (vRNAs) interact sequence-specifically with TEP1.
  • TEP1 possesses 16 WD40 repeats, suggesting a structural role in the eight-fold symmetric vault particle.

Conclusions:

  • TEP1 is a shared component of both vaults and telomerase.
  • Vaults do not possess telomerase activity, despite containing TEP1.
  • TEP1 may play a conserved role in ribonucleoprotein complex assembly, structure, or function.