Related Experiment Videos
Inhibition of Df-protease associated with allergic diseases by polyphenol
Y Noguchi1, K Fukuda, A Matsushima
1Toin Human Science and Technology Center, Department of Material Science and Technology, Toin University of Yokohama, Aoba-ku, Japan.
Abstract:
It was reported that Df-protease from house dust mite (Dermatophagoides farinae) catalyzes the activation of the kallikrein-kinin system in human plasma and is closely associated with mite-induced allergy. Therefore, to prevent the release of kinin by Df-protease, the inhibitory activity of polyphenols including catechins and flavonols was tested in vitro and in vivo. Among them, myricetin and epigallocatechin gallate (EGCg) effectively inhibited the amidase activity of Df-protease with K(i) values of 1 x 10(-)(8) and 6 x 10(-)(4) M, respectively. The kinin release in human plasma was extensively inhibited by the addition of EGCg in comparison with myricetin. Enhancement of vascular permeability in guinea pigs caused by Df-protease was markedly suppressed by EGCg.
Insights
Polyphenols like epigallocatechin gallate (EGCg) inhibit house dust mite (Dermatophagoides farinae) protease activity. This inhibition prevents kinin release and reduces mite-induced allergy symptoms.
Area of Science:
- Biochemistry
- Immunology
- Pharmacology
Background:
- House dust mite (Dermatophagoides farinae) protease (Df-protease) activates the kallikrein-kinin system.
- This activation is linked to mite-induced allergic reactions.
Purpose of the Study:
- To evaluate the inhibitory effects of polyphenols on Df-protease.
- To assess the potential of these compounds in preventing mite-induced allergy.
Main Methods:
- In vitro inhibition assays of Df-protease amidase activity using polyphenols.
- In vivo studies measuring vascular permeability changes in guinea pigs.
Main Results:
- Myricetin and epigallocatechin gallate (EGCg) demonstrated significant inhibition of Df-protease amidase activity.
- EGCg showed more extensive inhibition of kinin release in human plasma compared to myricetin.
- EGCg markedly suppressed Df-protease-induced enhancement of vascular permeability in vivo.
Conclusions:
- Polyphenols, particularly EGCg, are effective inhibitors of Df-protease.
- EGCg shows therapeutic potential for mitigating allergic responses triggered by house dust mites.