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Updated: Aug 14, 2026

A Straightforward Method for Glucosinolate Extraction and Analysis with High-pressure Liquid Chromatography (HPLC)
Published on: March 15, 2017
Fast HPLC for the analysis of oxygen heterocyclic compounds of citrus essential oils
I L Bonaccorsi1, H M McNair, L A Brunner
1Università di Messina, Dipartimento Farmaco-chimico Facoltà di Farmacia, Messina, Italy.
Abstract:
A fast HPLC method for the determination of the oxygen heterocyclic compounds of citrus essential oils was developed. Five different oils were analyzed under identical conditions, by reversed-phase HPLC with photodiode array detector, for a direct comparison of the composition of their oxygen heterocyclic fraction. Analysis time was 7 min. The oils analyzed were lemon, bergamot, mandarin, sweet orange, and bitter orange. The method developed is good for rapid screening or fingerprinting of these essential oils; a slightly slower method is recommended for higher resolution and better quantitative results.
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