Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Analysis of vervain flavonoids by HPLC/Diode array detector method. Its application to quality control.

P Valentão1, P B Andrade, F Areias

  • 1CEQUP/Laboratorio de Farmacognosia, Faculdade de Farmácia, Universidade do Porto, R. Aníbal Cunha, 4050 Porto, Portugal.

Journal of Agricultural and Food Chemistry
|December 20, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A sustainable approach by using microalgae to minimize the eutrophication process of Mar Menor lagoon.

The Science of the total environment·2020
Same author

Bioavailable phytoprostanes and phytofurans from Gracilaria longissima have anti-inflammatory effects in endothelial cells.

Food & function·2020
Same author

Plitidepsin to treat multiple myeloma.

Drugs of today (Barcelona, Spain : 1998)·2020
Same author

Optimization of Free Phytoprostane and Phytofuran Production by Enzymatic Hydrolysis of Pea Extracts Using Esterases.

Journal of agricultural and food chemistry·2020
Same author

Update on oxidative stress and inflammation in pregnant women, unborn children (nasciturus), and newborns - Nutritional and dietary effects.

Free radical biology & medicine·2019
Same author

Statement of Foliar Fertilization Impact on Yield, Composition, and Oxidative Biomarkers in Rice.

Journal of agricultural and food chemistry·2018

This study introduces a new HPLC method for analyzing seven flavonoids in vervain. Hispidulin and jaceosidin were identified as the predominant flavonoids in all tested vervain samples.

Area of Science:

  • Phytochemistry
  • Analytical Chemistry

Background:

  • Vervain (Verbena officinalis) is a medicinal plant with a complex phytochemical profile.
  • Flavonoids are a significant class of plant secondary metabolites with diverse biological activities.
  • Accurate quantification of flavonoids in medicinal plants is crucial for quality control and pharmacological studies.

Purpose of the Study:

  • To develop and validate a reversed-phase High-Performance Liquid Chromatography (HPLC) method for the simultaneous determination of seven specific flavonoids in vervain samples.
  • To establish a simple and efficient extraction technique for isolating flavonoids from vervain, minimizing interference from other plant compounds.
  • To analyze the flavonoid profile of various vervain samples and identify major and minor compounds.

Main Methods:

Related Experiment Videos

  • Development of a reversed-phase HPLC method utilizing a suitable stationary phase and mobile gradient.
  • Implementation of a simplified extractive technique involving ethyl ether extraction to purify the flavonoid fraction.
  • Analysis of multiple vervain samples to establish a common flavonoid pattern.

    • Main Results:

    • A robust reversed-phase HPLC procedure was successfully established for the accurate determination of seven flavonoids: luteolin, nepetin, hispidulin, jaceosidin, cirsimaritin, cirsilineol, and eupatorin.
    • The ethyl ether extraction method effectively removed interfering phenolic acids, yielding a purified flavonoid fraction suitable for HPLC analysis.
    • All analyzed vervain samples exhibited a consistent flavonoid profile, with hispidulin and jaceosidin identified as the major compounds and nepetin as the minor compound.

    Conclusions:

    • The developed reversed-phase HPLC method coupled with the simple extractive technique provides an efficient and reliable approach for analyzing flavonoids in vervain.
    • The consistent flavonoid pattern observed across different vervain samples, characterized by the dominance of hispidulin and jaceosidin, offers valuable insights into the plant's chemotaxonomy and quality assessment.
    • This method can be applied for the quality control and phytochemical standardization of vervain-based herbal products.