Effects of specific binding reactions on the partitioning behavior of biomaterials

Affinity partitioning is a special branch of biomaterials separations using aqueous two-phase systems. It combines the capability of diverse biomolecules to partition in aqueous two-phase systems using the principle of biorecognition. As a result, the macromolecule exhibiting affinity for a certain ligand is transferred to that phase where the ligand is present. This chapter describes the present status of the theoretical background of this approach and the properties of various natural and artificial compounds which act as affinity ligands in liquid-liquid systems. The affinity partitioning of proteins (enzymes and plasma proteins), cell membranes, cells, and nucleic acids are described as typical examples. The results are discussed in terms of theoretical understanding and practical application.