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Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

Chromosome measurement and sorting by flow systems

J W Gray, A V Carrano, L L Steinmetz

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1975
    PubMed

    Abstract:

    A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.

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