Related Experiment Video

Updated: Jul 16, 2026

Flow Cytometry Purification of Mouse Meiotic Cells
10:43

Flow Cytometry Purification of Mouse Meiotic Cells

Published on: April 15, 2011

Chromosome measurement and sorting by flow systems

J W Gray, A V Carrano, L L Steinmetz

    Proceedings of the National Academy of Sciences of the United States of America
    |April 1, 1975
    PubMed

    Abstract:

    A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3-1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high-resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. This novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.

    More Related Videos

    Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
    09:57

    Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

    Published on: July 12, 2018

    Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
    14:30

    Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

    Published on: August 7, 2021

    Related Experiment Videos

    Last Updated: Jul 16, 2026

    Flow Cytometry Purification of Mouse Meiotic Cells
    10:43

    Flow Cytometry Purification of Mouse Meiotic Cells

    Published on: April 15, 2011

    Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities
    09:57

    Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

    Published on: July 12, 2018

    Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors
    14:30

    Immunophenotyping and Cell Sorting of Human MKs from Human Primary Sources or Differentiated In Vitro from Hematopoietic Progenitors

    Published on: August 7, 2021

    Related Concept Videos

    Karyotyping01:17

    Karyotyping

    Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
    Flow Cytometry01:23

    Flow Cytometry

    The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
    In...

    Articles linked to this work by shared authors, journal, and citation graph.

    Psychostimulants for hypersomnia (excessive daytime sleepiness) in myotonic dystrophy.

    The Cochrane database of systematic reviews·2006

    The utility of presurgical CA125 to predict optimal tumor cytoreduction of epithelial ovarian cancer.

    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2006

    In utero angiopoietin-2 gene delivery remodels placental blood vessel phenotype: a murine model for studying placental angiogenesis.

    Molecular human reproduction·2005

    Placebo-controlled phase I/II studies of minocycline in amyotrophic lateral sclerosis.

    Neurology·2004

    The risk of nodal metastasis in early adenocarcinoma of the uterine cervix.

    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society·2004

    Riluzole for amyotrophic lateral sclerosis (ALS)/motor neuron disease (MND).

    Amyotrophic lateral sclerosis and other motor neuron disorders : official publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·2003

    Cerebellar microcircuits enable robust evidence-based decisions through cortico-cerebellar coupling.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    Indirect reciprocity with dual private assessment.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    Cell competition driven by secreted ligands: Modeling liver metastasis of colorectal cancer.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    Multilevel sex-influenced neurobiological signatures of early life adversity.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    SNHG26 orchestrates pro-fibrotic fibroblast maintenance via PTBP1-mediated alternative polyadenylation of AKT3 in dermal fibrosis.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    Synthetic DNA fragments as ultra-high-resolution multitracers to quantify transport behavior of micro- and nanoplastics in plant systems.

    Proceedings of the National Academy of Sciences of the United States of America·2026

    Next-generation multiplexed targeted proteomics quantifies post-translational modifications in disease and compound-protein interactions with high throughput.

    Nature communications·2026

    Effect of Processing Methods on the Chemical Composition and Cytotoxicity Against Esophageal Cancer Cells of Fresh Taraxacum mongolicum Hand.-Mazz. Leaves.

    Chemistry & biodiversity·2026

    Integrated strategy for multivariate quality description of Lonicera japonica by UHPLC-HRMS approach.

    Food chemistry: X·2026

    Enzymatic, Nondestructive Sequencing of 5-Methylcytosine with Direct Methylation Sequencing (DM-Seq).

    Methods in molecular biology (Clifton, N.J.)·2026

    Cocaine-Induced Mixed Diabetic Ketoacidosis-Hyperosmolar Hyperglycemic State Mimicking Acute Cholecystitis: A Case Report.

    Cureus·2026

    Estimation of DNA Methylation in Wheat Using Reverse Phase Ultra-High-Performance Liquid Chromatography Coupled with Mass Spectrometry During Leaf Rust Pathogenesis.

    Methods in molecular biology (Clifton, N.J.)·2026
    See all related articles
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies
    Jove
    Visualize
    Contact Us