Evaluation of the automated coagulation analyzer SYSMEX CA 6000

Area of Science:

• Clinical diagnostics
• Hematology
• Laboratory automation

Background:

• Coagulation testing is crucial for diagnosing and monitoring hemostatic disorders.
• Evaluating new analytical platforms is essential for improving laboratory efficiency and accuracy.
• The SYSMEX CA 6000 is a modern coagulation analyzer with advanced features like photooptical clot detection and a cap-piercing system.

Purpose of the Study:

• To evaluate the technical performance of the SYSMEX CA 6000 coagulation analyzer.
• To assess its accuracy and precision for standard coagulation tests and single factor activities.
• To investigate potential interferences and carryover effects.

Main Methods:

• Evaluation of SYSMEX CA 6000 for prothrombin time, activated partial thromboplastin time, thrombin time, fibrinogen, and antithrombin.
• Determination of coagulation single factor activities (V, VII, VIII, IX).
• Interference studies with lipemic, icteric, and hemolytic samples; carryover studies with heparinized plasma.

Main Results:

• Most intra- and interassay coefficients of variation (CV) were below 5%, with minor exceptions noted for specific parameters.
• Minor interferences were observed with lipemic, icteric, and hemolytic samples, comparable to mechanical clot detection methods.
• No carryover was detected between heparinized and normal plasma samples.
• Excellent correlation (r=0.977-0.993) was found between SYSMEX CA 6000 and STA for factor activity measurements.

Conclusions:

• The SYSMEX CA 6000 analyzer demonstrates satisfactory precision for routine and specialized coagulation testing.
• The integrated cap-piercing system does not adversely affect the analyzer's performance.
• The SYSMEX CA 6000 is a reliable instrument for hemostasis diagnostics.