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A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments
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Colorimetric Assay of Alditols in Complex Biological Samples.

Sánchez1

  • 1Instituto de la Grasa, CSIC, Avenida P. Garcia Tejero 4, 41012 Sevilla, Spain.

Journal of Agricultural and Food Chemistry
|February 7, 2001
PubMed
Summary

A new colorimetric assay accurately detects alditols, like mannitol, in biological samples. This rapid, cost-effective method uses simple reagents and achieves nanomolar sensitivity with minimal interference.

Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Alditol quantification is crucial for understanding various biological processes.
  • Existing methods for alditol determination can be complex, costly, or prone to interference.

Purpose of the Study:

  • To develop a simple, rapid, and sensitive colorimetric assay for alditol determination.
  • To minimize interference from other carbohydrates in biological extracts.

Main Methods:

  • Alditol oxidation using sodium periodate under acidic conditions.
  • Detection of formaldehyde byproduct via Hantzsch reaction with ammonia and acetylacetone.
  • Spectrophotometric measurement of the resulting colored heterocycle.

Main Results:

  • The assay demonstrates negligible interference from other carbohydrates.

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  • Detection limits reach the nanomolar range for mannitol and other alditols.
  • The method is rapid, completing within minutes.
  • The assay utilizes inexpensive and low-hazard chemicals.

    • Conclusions:

    • A convenient and sensitive colorimetric method for alditol analysis in complex biological samples has been established.
    • The assay is suitable for determining mannitol in plant extracts and validated against HPLC and enzymatic methods.