Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA image cytometry in MDS bone marrow smears.

S Widell1, R Hast, G Auer

  • 1Division of Cell and Molecular Analysis, Karolinska Hospital, Stockholm, Sweden. susanne.widell@swipnet.se

British Journal of Haematology
|November 11, 1999
PubMed
Summary

Myelodysplastic syndromes (MDS) often show lower nuclear DNA content in bone marrow cells. DNA hypodiploidy is a common feature in MDS, irrespective of cytogenetic findings.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Learning curves and breast cancer lymphatic mapping: institutional volume index.

The Journal of surgical research·2001
Same author

Encephalopathy associated with respiratory syncytial virus bronchiolitis.

Journal of child neurology·2001
Same author

Incidental discovery of pelvic cholelithiasis on diagnostic laparoscopy.

Surgical endoscopy·2001
Same author

Methylmercury and neurodevelopment: reanalysis of the Seychelles Child Development Study outcomes at 66 months of age.

JAMA·2001
Same author

Depression and self-reported functional status in older primary care patients.

The American journal of psychiatry·2001
Same author

A randomized, controlled trial of remacemide for motor fluctuations in Parkinson's disease.

Neurology·2001

Area of Science:

  • Hematology
  • Molecular Biology
  • Cytogenetics

Background:

  • Myelodysplastic syndromes (MDS) are a group of clonal hematopoietic stem cell disorders.
  • Characterizing DNA content abnormalities is crucial for understanding MDS pathogenesis.

Purpose of the Study:

  • To investigate nuclear DNA content using image cytometry (ICM) in myelodysplastic syndromes.
  • To compare DNA index (DI) in blasts/promyelocytes (bla/pro) between MDS patients and controls.
  • To correlate DNA content with cytogenetic data in MDS.

Main Methods:

  • Feulgen-stained bone marrow smears from 39 MDS patients and 8 controls were analyzed using ICM.
  • DNA index (DI) of blasts/promyelocytes was quantified.
  • Results were compared to normal cell types and available cytogenetic data.

Related Experiment Videos

Main Results:

  • MDS patients exhibited a significantly lower mean DI in bla/pro compared to controls (P < 0.01).
  • DNA aneuploidy was detected in 67% of MDS patients, with 59% showing DNA hypodiploidy.
  • DNA hypodiploidy was observed in MDS patients regardless of their karyotype status, including those with normal karyotypes.

Conclusions:

  • DNA hypodiploidy is a frequent finding in myelodysplastic syndromes.
  • The presence of DNA hypodiploidy in MDS does not appear to be directly related to cytogenetic abnormalities.