Jove
Visualize
Contact Us

Related Experiment Videos

Double embedding broad thin leaves in paraffin.

E T Graham1

  • 1Department of Ornamental Horticulture, Agricultural Experiment Station, The University of Tennessee, Knoxville 37901-1071, USA.

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
|November 11, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Somatic embryogenesis from immature embryos of redbud (Cercis canadensis).

Plant cell reports·2013
Same author

A novel frequency distribution selection method for efficient plate layout of a diverse combinatorial library.

Journal of chemical information and computer sciences·2001
Same author

Staining paraffin extracted, alcohol rinsed and air dried plant tissue with an aqueous mixture of three dyes.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·1998
Same author

Staining tomato fruit cuticle and exocarp tissues.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·1997
Same author

Inhibition of dipeptidyl peptidase IV (CD26) by peptide boronic acid dipeptides.

Journal of enzyme inhibition·1997
Same author

Kinetic characterization of human immunodeficiency virus type 1 protease: determination of inhibitor rate constants during dynamic monomer-dimer interconversion.

Archives of biochemistry and biophysics·1996
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

This study details a new paraffin embedding method for plant leaf specimens, ensuring perfect orientation for transverse sectioning. This technique improves tissue preparation for detailed microscopic analysis.

Area of Science:

  • Botany
  • Microscopy Techniques
  • Materials Science

Background:

  • Accurate specimen orientation is crucial for effective microscopic analysis in plant science.
  • Traditional paraffin embedding methods can present challenges in achieving precise orientation for delicate leaf tissues.
  • Optimized embedding protocols are essential for reproducible histological studies.

Purpose of the Study:

  • To develop and describe a novel double-embedding technique for plant leaf specimens using paraffin.
  • To ensure precise perpendicular orientation of leaf tissues for transverse sectioning.
  • To enhance the reliability and efficiency of histological sample preparation.

Main Methods:

  • Leaf specimens were fixed, dehydrated, and infiltrated with paraffin.

Related Experiment Videos

  • A two-step paraffin embedding process involving a base mold and a secondary embedding mold was employed.
  • Specimens were oriented perpendicular to the sectioning surface in the final paraffin block.

    • Main Results:

    • The double-embedding technique successfully oriented leaf specimens perpendicular to the sectioning surface.
    • The paraffin phases demonstrated good bonding, ensuring block integrity.
    • The method facilitates the creation of transverse ribbon sections.

    Conclusions:

    • This novel paraffin embedding method provides a reliable approach for preparing plant leaf specimens for detailed microscopic examination.
    • The technique ensures optimal orientation for transverse sectioning, advancing histological studies in botany.
    • The improved sample preparation contributes to more accurate and reproducible research outcomes.