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Structure of the murine E-selectin ligand 1 (ESL-1) gene and assignment to Chromosome 8
1Department of Human and Molecular Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.
Abstract:
A 150-kDa glycoprotein designated in the mouse as E-selectin ligand-1 (ESL-1; gene symbol Selel) was first isolated based on its ability to function as a ligand for E-selectin. The gene appears equivalent to that for membrane glycoprotein MG160 encoded in the human by the locus for Golgi apparatus protein 1 (GLG1). ESL-1 is also highly homologous to the chicken cysteine-rich fibroblast growth factor receptor (CFR). We describe the genomic structure and chromosomal localization of the Selel locus. The gene is encoded by 27 exons and extends over approximately 75 kb. It maps to murine Chromosome (Chr) 8 in a region homologous to human Chr 16q where the GLG1 locus maps, further indicating that Selel and GLG1 are mouse and human equivalents of the same gene.
Insights
Researchers identified the mouse E-selectin ligand-1 (ESL-1) gene, Selel, which is equivalent to the human GLG1 gene. This study details the genomic structure and chromosomal mapping of Selel, confirming its homology to human GLG1.
Area of Science:
- Molecular Biology
- Genomics
- Cell Biology
Background:
- E-selectin ligand-1 (ESL-1) is a 150-kDa glycoprotein in mice, identified by its function as an E-selectin ligand.
- The gene for ESL-1 (Selel) appears homologous to the human Golgi apparatus protein 1 (GLG1) gene, which encodes membrane glycoprotein MG160.
- ESL-1 also shows high homology to the chicken cysteine-rich fibroblast growth factor receptor (CFR).
Purpose of the Study:
- To describe the genomic structure of the mouse Selel locus.
- To determine the chromosomal localization of the Selel locus.
- To confirm the evolutionary relationship between mouse Selel and human GLG1.
Main Methods:
- Analysis of the Selel gene's exon-intron structure.
- Determination of the Selel gene's physical size.
- Chromosomal mapping of the Selel locus in mice using genetic markers.
- Comparative genomic analysis with human chromosome 16q.
Main Results:
- The Selel gene is composed of 27 exons and spans approximately 75 kb.
- The Selel locus maps to mouse Chromosome 8.
- This region on mouse Chromosome 8 is homologous to human Chromosome 16q, where the GLG1 locus is located.
Conclusions:
- The genomic structure and chromosomal localization of Selel provide further evidence that Selel and GLG1 represent the mouse and human equivalents of the same gene.
- This finding supports the conservation of this gene across species.
- The study enhances understanding of E-selectin ligand function and its genetic basis.