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Induction of apoptosis in macrophages by cationic liposomes
Y Aramaki1, S Takano, S Tsuchiya
1Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo, Japan. aramaki@ps.toyaku.ac.jp
Abstract:
The effects of liposomes on apoptosis in macrophages were evaluated from DNA content and DNA fragmentation. Cationic liposomes composed of different kinds of cationic lipids induced apoptosis in mouse splenic macrophages and the macrophage-like cell line, RAW264.7 cells. Generation of reactive oxygen radicals from macrophages treated with cationic liposomes was detected using flow cytometry, and further apoptosis was inhibited by the addition of oxidant scavenger, N-acetylcysteine. From these findings, the production of reactive oxygen species may be important in the regulation of apoptosis induced by cationic liposomes.
Insights
Cationic liposomes trigger apoptosis in macrophages, a process linked to reactive oxygen species generation. Inhibiting these species with N-acetylcysteine reduced liposome-induced cell death.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Liposomes are widely used drug delivery systems.
- Cationic liposomes have shown potential in various therapeutic applications.
- Understanding their interaction with immune cells is crucial.
Purpose of the Study:
- To investigate the impact of cationic liposomes on macrophage apoptosis.
- To explore the role of reactive oxygen species in this process.
Main Methods:
- Assessing DNA content and fragmentation in macrophages.
- Utilizing flow cytometry to detect reactive oxygen radical generation.
- Employing N-acetylcysteine as an oxidant scavenger.
Main Results:
- Cationic liposomes induced apoptosis in mouse splenic macrophages and RAW264.7 cells.
- Reactive oxygen species generation was observed in macrophages treated with cationic liposomes.
- N-acetylcysteine treatment inhibited liposome-induced apoptosis.
Conclusions:
- Cationic liposomes can induce apoptosis in macrophages.
- Reactive oxygen species production plays a significant role in cationic liposome-mediated apoptosis.
- Targeting reactive oxygen species may offer a strategy to mitigate liposome-induced cellular effects.