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Impact of cryopreservation on B cell chronic lymphocytic leukaemia phenotype
V Deneys1, V Thiry, N Hougardy
1Université Catholique de Louvain, University Hospital, Immunohaematology Laboratory, 1200, Brussels, Belgium. deneys@sang.ucl.ac.be
Background And Objectives:
Freezing is a practical approach for cell preservation for retrospective studies. The aim of this work was to check the cryopreservation impact on B cell chronic lymphocytic leukaemia phenotype.
Material And Methods:
Blood samples from 15 CLL patients were analyzed freshly and after freezing at -196 degrees C, without separation, and thawing. Results were compared by Student's paired t-test.
Results:
The phenotype of fresh CLL cells was as follows: CD19+, CD5+, faint CD20, CD23+/-, weak CD22 and sIg, CD37+, HLA-DR+, FMC7-. After cryopreservation, the percentage of CD5 and CD23 positive cells decreased, whereas HLA-DR positive cells increased moderately. The CLL Matutes's score was modified in 6 cases out of 15 (40%).
Conclusion:
Cryopreservation modifies B cell chronic lymphocytic leukaemia phenotype, by decreasing CD5 and CD23 expression.