Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carbohydrate moieties in human secretory component.

G J Hughes1, A J Reason, L Savoy

  • 1Department of Medical Biochemistry at the Medical Center, Faculty of Medicine, University of Geneva, 1 rue Michel-Servet, CH-1211, Geneva, Switzerland. hughes@cmu.unige.ch

Biochimica Et Biophysica Acta
|November 11, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Informing the use of a supplementary immunisation programme for the management of a community cluster of invasive meningococcal disease, Yorkshire, 2022.

Public health·2023
Same author

COVID-19 associated with universities in England, October 2020-February 2022.

Public health·2023
Same author

A protracted outbreak of difficult-to-treat resistant Pseudomonas aeruginosa in a haematology unit: a matched case-control study demonstrating increased risk with use of fluoroquinolone.

The Journal of hospital infection·2022
Same author

An outbreak of hepatitis A virus infection in a secondary school in England with no undetected asymptomatic transmission among students.

Epidemiology and infection·2022
Same author

A persistent recurring cluster of meticillin-resistant Staphylococcus aureus (MRSA) colonizations in a special care baby unit: a matched case-control study.

The Journal of hospital infection·2020
Same author

COVID-19: public health management of the first two confirmed cases identified in the UK.

Epidemiology and infection·2020
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Human secretory component (SC) glycosylation sites were identified and analyzed. The study found limited diversity in SC glycan structures, suggesting a potential role in mucosal surface protection.

Area of Science:

  • Biochemistry
  • Glycobiology
  • Immunology

Background:

  • Human secretory component (SC) is a key protein in mucosal immunity.
  • SC contains multiple N-linked glycosylation sites, but their glycan structures are not fully characterized.

Purpose of the Study:

  • To identify and characterize the N-linked glycans on human secretory component.
  • To investigate the diversity of glycan structures and their potential biological functions.

Main Methods:

  • Tryptic and Glu-C digests of human SC.
  • On-line HPLC-electrospray mass spectrometry for peptide analysis.
  • Fast atom bombardment mass spectrometry for oligosaccharide composition analysis after PNGase F treatment.

Main Results:

Related Experiment Videos

  • Seven N-linked glycosylation sites (Asn 65, 72, 117, 168, 403, 451, 481) were identified and confirmed to be fully glycosylated.
  • Major glycan structures identified were Fuc(2)Hex(5)HexNAc(4), Fuc(3)Hex(5)HexNAc(4), NeuAcFucHex(5)HexNAc(4), NeuAcFuc(2)Hex(5)HexNAc(4), NeuAc(2)Hex(5)HexNAc4, and NeuAc(2)FucHex(5)HexNAc(4).
  • Three of these oligosaccharides are also major glycans in human lactoferrin.

Conclusions:

  • Human SC exhibits a limited diversity of N-linked glycan structures.
  • These findings suggest a potential role for SC glycans in protecting mucosal surfaces.
  • The shared glycan structures with human lactoferrin may indicate conserved functional roles.