Related Experiment Videos
Distribution of glucose transporter isoform-3 and hexokinase I in the postnatal murine brain
H M Fields1, L Rinaman, S U Devaskar
1Division of Neonatology and Developmental Biology, Department of Pediatrics, University of Pittsburgh, Magee-Womens Research Institute, Pittsburgh, PA, USA.
Abstract:
The facilitative glucose transporter-3 (GLUT 3) and hexokinase I were examined in postnatal mouse brains using immunohistochemical methods. GLUT 3 demonstrated a polarized distribution limited to neuronal processes of most anatomical regions except the suprachiasmatic nucleus and the cerebellum, where GLUT 3 expression was limited to neuronal cell somata. In contrast, hexokinase I was observed in the cytoplasm of neuronal and non-neuronal (subependymal and choroid plexus epithelial) cell bodies in all regions. In general, while the spatial distribution of GLUT 3 and hexokinase I did not change with age, a temporal increase in intensity was noted in all regions except for the decline in suprachiasmatic nuclear GLUT 3 immunoreactivity.