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Localisation of the cell wall-associated phosphodiesterase PhoD of Bacillus subtilis
1Institute of Molecular Biology, Jena University, Winzerlaer Str. 10, D-07745, Jena, Germany. jmueller@imb-jena.de
FEMS Microbiology Letters
|November 11, 1999
Abstract:
The localisation of phosphate-starvation-induced phosphodiesterase PhoD from Bacillus subtilis was studied by analysing processing, release and immunogold labelling of the sections. Although the processing of the pre-protein was extremely slow, the major fraction of PhoD could be detected at the surface of the cell wall. The results indicate that inefficient processing of the translocated pre-protein keeps PhoD in a cell wall-associated location. The uncleaved signal peptide might function as a membrane anchor.