Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Biosensor analysis of the interleukin-2 receptor complex.

S F Liparoto1, T L Ciardelli

  • 1Department of Pharmacology and Toxicology, Dartmouth Medical School, Hanover, NH 03755, USA.

Journal of Molecular Recognition : JMR
|November 11, 1999
PubMed
Summary

Surface plasmon resonance (SPR) biosensor technology offers a powerful method for studying macromolecular interactions, particularly the complex interleukin-2 receptor (IL-2R) system. This advanced technique provides quantitative insights into binding characteristics previously limited by other methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased endosomal sorting of ligand to recycling enhances potency of an interleukin-2 analog.

The Journal of biological chemistry·2000
Same author

Solution assembly of the pseudo-high affinity and intermediate affinity interleukin-2 receptor complexes.

Protein science : a publication of the Protein Society·1999
Same author

Partial signaling by cytokines: cytokine regulation of cell cycle and Fas-dependent, activation-induced death in CD4+ subsets.

Cellular immunology·1998
Same author

Alzheimer disease hyperphosphorylated tau aggregates hydrophobically.

Synapse (New York, N.Y.)·1997
Same author

Structural modifications of interleukin-2 at positions 47 and 65.

Biochemical and biophysical research communications·1997
Same author

The functional display of interleukin-2 on filamentous phage.

Archives of biochemistry and biophysics·1997

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Studying the interleukin-2 receptor (IL-2R) system previously relied on various biochemical and biological techniques.
  • These methods, including site-directed mutagenesis and radioligand binding, offered insights into structure-activity relationships but had limitations.
  • Cell-based assays struggled to provide quantitative data on IL-2R subclasses due to system complexity.

Purpose of the Study:

  • To demonstrate the feasibility and utility of Surface Plasmon Resonance (SPR) biosensor technology for analyzing complex receptor systems.
  • To investigate the ligand binding characteristics of novel, pre-assembled IL-2R coiled-coil complexes using Biacore analysis.
  • To establish SPR as a powerful tool for quantitative analysis of hematopoietic receptor systems.

Main Methods:

Related Experiment Videos

  • Surface Plasmon Resonance (SPR) biosensor analysis, including Biacore instrumentation.
  • Site-directed mutagenesis, equilibrium and kinetic radioligand binding assays.
  • Competitive biological assays and analysis of individual IL-2R subunits and heteromeric complexes.

Main Results:

  • Initial studies using Biacore analysis confirmed the utility of SPR for investigating IL-2R ligand binding, despite early instrumentation limitations.
  • Advancements in SPR instrumentation and data analysis enabled detailed kinetic analyses of IL-2R subunits and complexes.
  • SPR biosensor analysis, combined with other methods, proved effective for characterizing complex hematopoietic receptor systems.

Conclusions:

  • SPR biosensor technology is a significant advancement for studying macromolecular interactions, especially complex receptor systems like IL-2R.
  • The method overcomes limitations of traditional assays, providing quantitative binding characteristics.
  • SPR, integrated with established techniques, is a powerful tool for analyzing complex hematopoietic receptor systems.