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Exploration of the association between apoptosis and cleft palate
1Department of Oral and Maxillofacial Surgery, College of Stomatology, Sun Yat-sen University of Medical Sciences (SUMS), Guangzhou, P. R. China.
Objective:
To explore the significance and effect of apoptosis in the development of cleft palate induced by dexamethasone (DEX).
Methods:
Various doses of DEX (10-50 mg/kg) were injected intraperitoneally into Kunming mice on gestation day (GD) 12. The cranio-maxillary sections of samples were labeled by HRP-avidin-biotin-dUTP in situ end-labeling (ISEL) and were observed morphologically and quantitatively under a light microscope and electron microscope.
Results:
ISEL positive cells were found in treatment groups. The means of sums and cleft palate incidence have a positive correlation with the doses of DEX (r = 0.9911/0.9978).
Conclusion:
A relationship exists between an increased level of apoptosis and the development of cleft palate induced by DEX.