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An improved transposon for the halophilic archaeon Haloarcula hispanica
W G Woods1, K Ngui, M L Dyall-Smith
1Department of Microbiology, University of Melbourne, Parkville, Victoria 3052, Australia.
Journal of Bacteriology
|November 13, 1999
Abstract:
An improved transposon (ThD73) for Haloarcula hispanica is described. Based on the halobacterial insertion sequence ISH28, it showed little target sequence specificity but was biased toward a lower G+C content. Twenty randomly selected ThD73 mutants were analyzed, and the DNA flanking their insertions revealed several recognizable sequences, including two (unrelated) ISH elements.