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Updated: Aug 7, 2026

Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
Activation of a meiotic checkpoint regulates translation of Gurken during Drosophila oogenesis
1HHMI, Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.
Abstract:
The genes okra and spindle-B act during meiosis in Drosophila to repair double-stranded DNA breaks (DSBs) associated with meiotic recombination. Unexpectedly, mutations in these genes cause dorsoventral patterning defects during oogenesis. These defects result from a failure to accumulate Gurken protein, which is required to initiate dorsoventral patterning during oogenesis. Here we find that the block in Gurken accumulation in the oocyte cytoplasm reflects activation of a meiotic checkpoint in response to the persistence of DSBs in the nucleus. We also show that Vasa is a target of this meiotic checkpoint, and so may mediate the checkpoint-dependent translational regulation of Gurken.
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