Related Experiment Videos
Influence of sample size on image cytometry of DNA ploidy measurements
1Pôle traitement et Analyse d'Images de Basse-Normandie, F. Baclesse Cancer Center, University of Caen (UMR CNRS 6551), France.
Objective:
To assess the number of nuclei required for significant image cytometry DNA ploidy measurements on one archival case of breast cancer.
Study Design:
From one case of aneuploid DNA breast cancer, 18 subsets made up of 152-1,524 for the whole population of undamaged nuclei and made up of 74-735 epithelial nuclei had DNA measured. DNA ploidy type and five DNA ploidy indices, allowing DNA ploidy histogram interpretation were evaluated on each population.
Results:
Three hundred nuclei were always sufficient for DNA typing, whereas reliable results for DNA ploidy indices required at least 750 nuclei.
Conclusion:
To DNA measure the above number of nuclei, fully automated image cytometry DNA ploidy measurements are required.