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Updated: Aug 14, 2026

Primary Human Bronchial Epithelial Cells Grown from Explants
Published on: March 26, 2010
Roundness of epithelial nuclei in bronchial mucosa
Z Mijović1, C Kutlesić, D Mihailović
1Medical Faculty, Institute of Pathology, University of Nis, Serbia, Yugoslavia. mihapat@kalca.junis.ni.ac.yu
Objective:
To estimate the shape of epithelial nuclei in bronchial mucosa.
Study Design:
Bronchoscopic mucosal samples from 12 patients with chronic bronchitis and 14 patients with squamous cell bronchial carcinoma were routinely processed and stained with the hematoxylin and eosin method. Fields with stratification and squamous metaplasia were selected. The roundness of epithelial nuclei was estimated using Lucia, version 3.51 ab, with an objective of 40:1 (NA = 0.65) and final magnification of 1,900:1. The images were manually edited. Roundness was calculated from area and perimeter.
Results:
The mean roundness in stratification and squamous metaplasia in bronchial mucosa from carcinoma patients (0.90 +/- 0.04) was significantly greater than in bronchial mucosa from chronic bronchitis patients (0.80 +/- 0.006) (P < .05).
Conclusion:
Our results indicate that cell nuclei in stratification and squamous metaplasia in patients without bronchial cancer are more elongated than nuclei found under the same conditions in patients with squamous cell carcinoma.
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