Alpha-trifluoromethylated acyloins induce apoptosis in human oral tumor cell lines
M Kawase1, H Sakagami, K Kusama
1Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan.
Bioorganic & Medicinal Chemistry Letters
|November 24, 1999
Abstract:
Cytotoxic activity of newly synthesized trifluoromethyl ketones and related compounds was studied using two human oral tumor cell lines (HSG and HSC-2). Among them, alpha-trifluoromethylacyloins (1 and 2) were found to induce apoptotic cell death, as judged by the terminal deoxynucleotidyl transferase (TdT) dUTP nick end-labeling (TUNEL) method which detects DNA nick or fragments. Furthermore, the cytoplasm of 1 or 2 treated HSG cells was stained by M30 monoclonal antibody, which detects the product resulting from the cleavage of cytokeratin 18 by activated caspase.
