Jove
Visualize
Contact Us

Related Experiment Videos

Expression and sequence requirements for nitrite reductase co-suppression.

P Crété1, H Vaucheret

  • 1Laboratoire de Biologie Cellulaire, INRA, Versailles, France.

Plant Molecular Biology
|November 24, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Complete nucleotide sequence of the two homeologous tobacco nitrate reductase genes.

Plant molecular biology·2013
Same author

Asymmetric hybridization in Nicotiana by "gamma fusion" and progeny analysis of self-fertile hybrids.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Agrobacterium mediated transfer of a mutant Arabidopsis acetolactate synthase gene confers resistance to chlorsulfuron in chicory (Cichorium intybus L.).

Plant cell reports·2013
Same author

Selection for spontaneous tomato haploids using a conditional lethal marker.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Nitrite reductase silencing as a tool for selecting spontaneous haploid plants.

Plant cell reports·2013
Same author

Embryogeny of hydrophyllaceas; Development of the embryo in Nemophila insignis Benth.

Comptes rendus hebdomadaires des seances de l'Academie des sciences·2010
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Chimeric tobacco nitrite reductase Nii1 transgenes lacking specific 5' and 3' cDNA ends did not trigger gene co-suppression. Results suggest a metastable epigenetic modification is required for this silencing mechanism.

Area of Science:

  • Plant Molecular Biology
  • Gene Silencing Mechanisms
  • Transgenic Plant Technology

Background:

  • Previous work showed full-length tobacco nitrite reductase (Nii1) cDNA triggers co-suppression of endogenous Nii genes in some transformants.
  • Co-suppression is a phenomenon where homologous genes are silenced, often involving post-transcriptional gene silencing (PTGS).

Purpose of the Study:

  • To investigate the role of specific cDNA regions (5' and 3' ends) of tobacco Nii1 in triggering gene co-suppression.
  • To determine if these regions are targets for homology-dependent RNA degradation.
  • To explore the potential for epigenetic modifications in co-suppression release.

Main Methods:

  • Generation and transformation of chimeric Nii1-uidA, uidA-Nii1, and Nii1-uidA-Nii1 transgenes in tobacco.

Related Experiment Videos

  • Crossing of chimeric transgenes into existing co-suppressed lines.
  • Analysis of gene expression and silencing in transgenic and F2 progeny.
  • Regeneration of plants from untransformed leaf discs.

    • Main Results:

    • Chimeric transgenes lacking 5' and/or 3' Nii1 cDNA ends did not induce co-suppression.
    • These chimeric transgenes were not silenced when introduced into already co-suppressed lines.
    • Co-suppression was unexpectedly released in a double transformant and a regenerated plant, suggesting a metastable epigenetic modification.
    • Analysis of Nii mRNA levels indicated transcription above a threshold is necessary for co-suppression.

    Conclusions:

    • The 5' and 3' ends of Nii1 cDNA are insufficient to trigger co-suppression and are not targets for RNA degradation.
    • A metastable epigenetic modification, potentially linked to transgene transcription levels, plays a role in the regulation of co-suppression.
    • This study provides insights into the complex mechanisms regulating gene silencing in transgenic plants.