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Related Experiment Videos

CD2 sets quantitative thresholds in T cell activation.

M F Bachmann1, M Barner, M Kopf

  • 1Basel Institute for Immunology, CH 4005 Basel, Switzerland. bachmann@bii.ch

The Journal of Experimental Medicine
|November 24, 1999
PubMed
Summary

CD2 enhances T cell activation by improving adhesion and signaling. Its absence impairs early T cell responses, demonstrating its role in fine-tuning immune thresholds.

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Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Medicine

Background:

  • CD2 is highly expressed on T cells and is proposed to enhance T cell-antigen presenting cell (APC) adhesion and costimulate T cell activation.
  • Understanding the precise role of CD2 in T cell activation is crucial for comprehending immune responses.

Purpose of the Study:

  • To analyze the role of CD2 in T cell activation using genetically modified mouse models.
  • To investigate the contribution of CD2 in conjunction with other adhesion molecules like LFA-1/ICAM-1.

Main Methods:

  • Utilized CD2-deficient mice crossed with transgenic mice expressing a T cell receptor specific for LCMV-derived peptide p33.
  • Assessed T cell activation through in vitro dose-response curves and in vivo proliferation assays.
  • Analyzed early T cell activation steps including conjugate formation, TCR triggering, and calcium fluxes.

Main Results:

  • Absence of CD2 shifted the p33-specific dose-response curve in vitro by a factor of 3-10.
  • CD2 and LFA-1/ICAM-1 interactions were found to facilitate T cell activation additively.
  • T cell activation at low antigen density was significantly impaired in CD2-deficient T cells, affecting early signaling events.

Conclusions:

  • CD2 plays a critical role in setting quantitative thresholds for T cell activation.
  • CD2 fine-tunes T cell activation both in vitro and in vivo, particularly under conditions of low antigen availability or cross-priming.
  • The findings highlight CD2's importance in modulating the sensitivity and magnitude of T cell responses.

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