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Related Experiment Videos

Morphological study on pigmented cells in the horse testis.

H Murabayashi1, E Hondo, N Kitamura

  • 1Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

The Journal of Veterinary Medical Science
|November 24, 1999
PubMed
Summary

Horse testes weight changes during development, with Leydig cell numbers decreasing and pigment appearing. This pigment is identified as ceroid-like, likely formed by macrophages digesting Leydig cells.

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Area of Science:

  • Veterinary Anatomy
  • Reproductive Biology
  • Cellular Histology

Background:

  • Horse testicular weight undergoes significant changes during development.
  • A decrease in Leydig cell count correlates with the appearance of interstitial pigments.
  • Understanding these pigments is crucial for comprehending testicular aging and function.

Purpose of the Study:

  • To characterize the pigments present in horse testicular interstitial tissues.
  • To investigate the cellular origin and nature of these pigmented granules.
  • To elucidate the relationship between Leydig cells, macrophages, and pigment formation.

Main Methods:

  • Histochemical staining techniques were employed to analyze pigment characteristics.
  • Ultrastructural examination provided insights into the cellular location and morphology of pigments.

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  • Acid phosphatase activity was assessed to identify cellular involvement.

    • Main Results:

    • Pigmented granules exhibited histological and histochemical properties consistent with ceroid or ceroid-like pigment.
    • Cells containing pigment showed positive acid phosphatase reactions, indicating lysosomal activity.
    • Ultrastructural analysis revealed abundant lysosomes within pigmented cells.

    Conclusions:

    • The observed pigment is likely a ceroid-like substance.
    • Macrophages appear to be involved in the formation of this pigment.
    • Macrophages may phagocytize degenerating Leydig cells, storing digested material as ceroid-like pigment within their lysosomes.