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Comparative study of polyphenol scavenging activities assessed by different methods.
N Saint-Cricq De Gaulejac1, C Provost, N Vivas
1Demptos cooperage, posted to Faculté d'OEnologie, Laboratoire de Chimie Appliquée, Université Victor Segalen Bordeaux II, Talence, France.
Journal of Agricultural and Food Chemistry
|November 24, 1999
Summary
Grape procyanidins are potent antioxidants, effectively scavenging free radicals. Their antioxidant capacity, superior to common antioxidants like ascorbic acid, depends on their unique chemical structures.
Area of Science:
- Food Chemistry
- Biochemistry
- Natural Product Chemistry
Background:
- Phenolic compounds, including procyanidins, are known for their antioxidant properties.
- Understanding the radical scavenging potential of grape-derived procyanidins is crucial for food and health applications.
Purpose of the Study:
- To investigate the free radical scavenging activity of procyanidin solutions.
- To determine the influence of chemical and stereochemical structures on antioxidant potential.
- To compare the efficacy of grape procyanidins with commercial antioxidants.
Main Methods:
- Enzymatic method to assess superoxide anion radical (O(2)(*)(-)) scavenging.
- Chemical method using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH(*)).
- Immunochemical method to evaluate scavenging of hydroxyl radical (HO(*))-induced DNA damage.
- Isolation of oligomeric procyanidins from grapes and wines using chromatography.
Main Results:
- Procyanidins demonstrated significant free radical scavenging activity, with EC(50) values determined.
- Antioxidant potential was found to be highly dependent on the chemical and stereochemical structures of procyanidins.
- Oligomeric procyanidins from grapes and wines exhibited potent scavenging even at low concentrations.
- Grape procyanidins showed superior activity compared to ascorbic acid and gallic acid.
Conclusions:
- Procyanidins are highly effective natural antioxidants with potential applications in food and health.
- The structural characteristics of procyanidins are key determinants of their radical scavenging efficacy.
- Grape and wine procyanidins represent a valuable source of potent antioxidants.