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Xenopus laevis as a Model to Identify Translation Impairment
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Xenopus laevis as a Model to Identify Translation Impairment

Published on: September 27, 2015

c-mos and cdc2 cooperate in the translational activation of fibroblast growth factor receptor-1 during Xenopus oocyte

P A Culp1, T J Musci

  • 1Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California School of Medicine, San Francisco, California 94143-0556, USA.

Molecular Biology of the Cell
|November 17, 1999
PubMed

Insights

Cell cycle regulators c-mos and cdc2 cooperate to activate translation of specific maternal mRNAs during Xenopus oocyte maturation. They interact with distinct mRNA elements, with cdc2 acting independently of polyadenylation and c-mos requiring it.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • Maternal mRNAs are translationally regulated during Xenopus oocyte maturation.
  • Specific cellular events trigger the translational recruitment of individual maternal mRNAs.

Purpose of the Study:

  • Investigate how cell cycle regulators c-mos and cdc2 activate translation of the FGF receptor-1 (XFGFR) maternal mRNA.
  • Identify the mechanisms by which c-mos and cdc2 control XFGFR translation.

Main Methods:

  • Injection of recombinant cdc2/cyclin B and mos RNA into Xenopus oocytes.
  • Depletion of c-mos and blocking of cdc2 activity.
  • Use of injected reporter RNAs to analyze translational control elements.
  • Assessment of polyadenylation status.

Main Results:

  • Both c-mos and cdc2 activate XFGFR translation.
  • cdc2 alone derepresses an XFGFR 3' untranslated region inhibitory element.
  • c-mos requires poly(A) extension for translational activation.
  • A novel inhibitory element responsive to both c-mos and cdc2 was identified.

Conclusions:

  • c-mos and cdc2 cooperate to regulate XFGFR maternal mRNA translation during oocyte maturation.
  • These regulators utilize distinct mechanisms involving mRNA elements and polyadenylation.
  • The findings reveal a coordinated control of maternal mRNA translation by meiotic cell cycle effectors.

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