Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Keratocytes generate traction forces in two phases.

K Burton1, J H Park, D L Taylor

  • 1Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA. K.Burton@cmu.edu

Molecular Biology of the Cell
|November 17, 1999
PubMed
Summary

Researchers precisely measured cell forces using nanonewton precision, revealing a two-phase mechanism for cell motility. This study details how forces in the lamellipodium and cell body drive cell movement and adhesion dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Composition of soil <i>Frankia</i> assemblages across ecological drivers parallels that of nodule assemblages in <i>Alnus incana</i> ssp. <i>tenuifolia</i> in interior Alaska.

Ecology and evolution·2024
Same author

Leptin activation of dorsal raphe neurons inhibits feeding behavior.

bioRxiv : the preprint server for biology·2023
Same author

Symbiotic germination and development of myco-heterotrophic plants in nature: ontogeny of Corallorhiza trifida and characterization of its mycorrhizal fungi.

The New phytologist·2021
Same author

ARCHITECTURAL PATTERNS FOR DIFFERENTIAL DIAGNOSIS OF PROLIFERATIVE BREAST LESIONS FROM HISTOPATHOLOGICAL IMAGES.

Proceedings. IEEE International Symposium on Biomedical Imaging·2017
Same author

Pre-clinical and clinical investigations of metabolic zonation in liver diseases: The potential of microphysiology systems.

Experimental biology and medicine (Maywood, N.J.)·2017
Same author

Trophic structure of a coastal fish community determined with diet and stable isotope analyses.

Journal of fish biology·2016

Area of Science:

  • Cell Biology
  • Biophysics
  • Mechanobiology

Background:

  • Cell motility is crucial for biological processes.
  • Understanding the forces cells generate is key to deciphering motility mechanisms.

Purpose of the Study:

  • To precisely measure forces generated by motile cells.
  • To elucidate the mechanical basis of cell motility and adhesion dynamics.

Main Methods:

  • Utilized nanonewton precision force measurements.
  • Employed differential interference contrast microscopy for visualizing deformations.
  • Used interference reflection microscopy to identify cell-substratum adhesions.

Main Results:

  • Measured forces ranging from nanonewtons (lamellipodium) to hundreds of nanonewtons (cell body).

Related Experiment Videos

  • Observed centripetal forces applied by lamellipodia and lateral forces at the cell body boundary.
  • Demonstrated increased stress during cell retraction due to rapid decrease in contact area.

    • Conclusions:

    • Proposed a two-phase model for cell motility.
    • Phase 1: Lamellipodial forces drive forward movement of the cell body.
    • Phase 2: Flank forces aid rearward margin advancement and lateral forces facilitate adhesion detachment.