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Transformations of codeine to important semisynthetic opiate derivatives by Pseudomonas putida m10
D L Lister1, G Kanungo, D A Rathbone
1Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, UK.
FEMS Microbiology Letters
|November 24, 1999
Abstract:
A biotransformation mixture which contained codeine and washed cells of Pseudomonas putida M10 gave rise to a number of transformation products that are of clinical importance which included hydrocodone, dihydrocodeine and 14beta-hydroxycodeine. Incubations with the same organism and codeinone gave rise to 14beta-hydroxycodeinone and 14beta-hydroxycodeine. Cell-free extracts and membrane fractions of P. putida M10 were shown to catalyse the 14beta-hydroxylation of codeinone. In addition, the potent analgesic oxycodone was shown to be produced from 14beta-hydroxycodeinone.