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Measurement of protein synthesis in human tissues by the flooding method
1Department of Surgery, State University of New York, Stony Brook 11794-8191, USA. garlick@surg.som.sunysb.edu
Current Opinion in Clinical Nutrition and Metabolic Care
|November 24, 1999
Abstract:
The flooding procedure for measuring protein synthesis in individual tissues with amino acids labelled with stable isotopes is explained, and its advantages for studies in humans are described. The application of this method for investigating the effects of nutrition in healthy volunteers and sick patients is illustrated with examples of studies on skeletal muscle, liver (including albumin) and lymphocytes.