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Functional Cloning Using a Xenopus Oocyte Expression System
Published on: January 30, 2016
Cloning of cDNAs encoding human synapsins IIa and IIb
B Porton1, H T Kao, P Greengard
1Laboratory of Molecular and Cellular Neuroscience, The Rockefeller University, New York, New York 10021, USA.
DNA Sequence : the Journal of DNA Sequencing and Mapping
|November 24, 1999
Summary
Researchers cloned and sequenced human synapsins IIa and IIb, crucial neuronal phosphoproteins involved in neurotransmitter release. Understanding these proteins is vital for investigating neuropsychiatric diseases.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Synapsins are neuronal phosphoproteins associated with synaptic vesicles.
- They play roles in neurotransmitter release and synaptogenesis.
- Aberrant synapsin function is linked to neuropsychiatric diseases.
Purpose of the Study:
- To characterize human synapsins IIa and IIb.
- To evaluate the role of synapsins in human neuropathology.
Main Methods:
- Cloning of human synapsin II gene.
- Sequencing of human synapsins IIa and IIb.
Main Results:
- Successful cloning and sequencing of human synapsins IIa and IIb.
- Human synapsins IIa and IIb fit the established synapsin domain model.
- Protein domains A, C, and E were identified as the most conserved.
Conclusions:
- Human synapsins IIa and IIb have been characterized.
- This characterization provides a basis for studying their role in neuropathology.
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