Related Experiment Videos
Structure and sequence of the gene encoding human keratocan.
E S Tasheva1, J L Funderburgh, M L Funderburgh
1Division of Biology, Kansas State University, Manhattan 66506-4901, USA. est@ksu.edu
DNA Sequence : the Journal of DNA Sequencing and Mapping
|November 24, 1999
Summary
Researchers identified the human keratocan (Kera) gene, crucial for corneal transparency. Its conserved sequence across mammals suggests a vital role in maintaining eye clarity.
Area of Science:
- Ophthalmology
- Molecular Biology
- Genetics
Background:
- Keratocan is a key proteoglycan in the cornea.
- Corneal transparency is vital for vision.
Purpose of the Study:
- To sequence and characterize the human keratocan (Kera) gene.
- To understand the role of keratocan in corneal function.
Main Methods:
- cDNA and genomic cloning
- Gene sequencing
- Sequence analysis
Main Results:
- The human Kera gene spans 7.65 kb and has three exons.
- An open reading frame encodes a 352 amino acid protein.
- Keratocan shows high sequence identity across mammalian species.
Conclusions:
- The Kera gene's structure and conserved protein sequence suggest its importance.
- Restricted expression in the cornea points to a role in maintaining corneal transparency.