Related Experiment Video

Updated: Oct 18, 2026

Establishment and Characterization of UTI and CAUTI in a Mouse Model
08:40

Establishment and Characterization of UTI and CAUTI in a Mouse Model

Published on: June 23, 2015

Leuconostoc pseudomesenteroides as a cause of nosocomial urinary tract infections

E A Cappelli1, R R Barros, T C Camello

  • 1Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro 20551, Brazil.

Journal of Clinical Microbiology
|November 24, 1999
PubMed

Insights

This study characterized five clinical isolates of Leuconostoc pseudomesenteroides causing hospital-acquired urinary tract infections. Evidence suggests these vancomycin-resistant opportunistic pathogens may be transmitted within healthcare settings.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Clinical Science

Background:

  • Nosocomial infections pose a significant threat in healthcare settings.
  • Urinary tract infections (UTIs) are common hospital-acquired infections.
  • Emergence of antibiotic-resistant pathogens complicates treatment strategies.

Purpose of the Study:

  • To conduct phenotypic and genotypic characterization of Leuconostoc pseudomesenteroides clinical isolates.
  • To investigate the antibiotic susceptibility profiles of these isolates.
  • To determine the potential for nosocomial transmission among these strains.

Main Methods:

  • Phenotypic characterization including antibiotic susceptibility testing.
  • Genotypic characterization using pulsed-field gel electrophoresis (PFGE) of chromosomal DNA after SmaI digestion.
  • Analysis of five clinical isolates from nosocomial UTIs.

Main Results:

  • All isolates were susceptible to chloramphenicol, clindamycin, erythromycin, gentamicin, and tetracycline.
  • All isolates exhibited resistance to nalidixic acid, norfloxacin, and vancomycin.
  • PFGE analysis revealed a clonal relationship among the isolates, suggesting a common source or transmission.

Conclusions:

  • Leuconostoc pseudomesenteroides can act as an opportunistic pathogen in hospital-acquired UTIs.
  • The isolates demonstrated a concerning resistance pattern, including to vancomycin.
  • Genotypic data support the possibility of nosocomial transmission of this pathogen.

More Related Videos

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice08:53

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Published on: December 4, 2020

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection07:57

An In Vitro Bladder Model of Catheter-Associated Urinary Tract Infection

Published on: June 24, 2025

Related Concept Videos

Urinary Tract Infection II: Pathophysiology01:25

Urinary Tract Infection II: Pathophysiology

The pathophysiology of urinary tract infections (UTIs) encompasses several progressive stages, beginning with bacterial colonization and culminating in potential systemic complications if untreated. UTIs are primarily initiated by bacteria, such as Escherichia coli, which often originate from the gastrointestinal tract and migrate to the urinary system through the periurethral area. This migration can occur via several routes, including improper hygiene practices, sexual activity, or...
Urinary Tract Infection I: Introduction01:26

Urinary Tract Infection I: Introduction

Urinary tract infections (UTIs) impact various parts of the urinary system, including the kidneys, ureters, bladder, and urethra. These infections are generally bacterial, with Escherichia coli being the most common causative agent, often originating from the gastrointestinal tract. However, other bacteria, such as Staphylococcus saprophyticus, Klebsiella pneumoniae, and Proteus mirabilis, are also known to cause UTIs. The type, location, and underlying complexity of the UTI guide both...
Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
Acute Pyelonephritis I: Introduction01:27

Acute Pyelonephritis I: Introduction

Pyelonephritis is a bacterial infection that primarily affects the renal parenchyma and collecting system, including the renal pelvis, tubules, and interstitial tissue of one or both kidneys. It can be classified as either acute—a sudden, severe infection—or chronic, which refers to long-term or recurrent kidney infections.The primary cause of acute pyelonephritis (APN) is bacterial infection, with Escherichia coli accounting for approximately 70-80% of cases. Other bacteria, such as Proteus,...
Microbiota of the Urogenital Tract01:28

Microbiota of the Urogenital Tract

The human urogenital system, once thought to be sterile in healthy individuals, is now recognized as a complex microbial habitat. Advancements in molecular sequencing techniques have revealed that even in healthy adults, the kidneys and bladder harbor microbial populations similar to those found in the distal urethra, albeit in much lower abundance. These resident microorganisms, while generally innocuous, can become opportunistic pathogens under conditions that alter the urogenital...
Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...