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Related Experiment Videos

Sequence analysis of a small cryptic plasmid isolated from Streptococcus suis serotype 2.

D Takamatsu1, M Osaki, T Sekizaki

  • 1National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.

Current Microbiology
|November 24, 1999
PubMed
Summary

A novel plasmid, pSSU1, was identified in Streptococcus suis. This cryptic plasmid utilizes a rolling circle replication mechanism, suggesting a conserved replication strategy among related plasmids.

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Area of Science:

  • Bacteriology
  • Molecular Biology
  • Plasmid Biology

Background:

  • Streptococcus suis serotype 2 is a significant pathogen.
  • Cryptic plasmids play roles in bacterial adaptation and evolution.
  • Understanding plasmid replication mechanisms is crucial for genetic studies.

Purpose of the Study:

  • To characterize the genetic makeup and replication mechanism of the novel plasmid pSSU1.
  • To identify potential functional elements within the pSSU1 sequence.

Main Methods:

  • Plasmid isolation from Streptococcus suis strain DAT1.
  • Complete DNA sequencing of the pSSU1 plasmid.
  • Bioinformatic analysis of open reading frames (ORFs) and regulatory sequences.
  • Comparative analysis with known plasmid families.

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Main Results:

  • The pSSU1 plasmid is 4975 bp with six major ORFs.
  • ORF1, ORF2, and ORF5 show homology to proteins involved in replication and mobilization (CopG, RepB, Mob) of the pMV158 family.
  • Putative double-strand origin (DSO) and single-strand origin (SSO) sequences indicative of rolling circle replication were identified.
  • Homology to countertranscribed RNA (ctRNA) and conserved C-terminal sequences in Rep proteins suggest conserved regulatory mechanisms.

Conclusions:

  • The pSSU1 plasmid likely replicates via the rolling circle mechanism.
  • pSSU1 shares conserved features with the pMV158 plasmid family, indicating potential functional similarities.
  • Further studies are warranted to elucidate the function of unknown ORFs and the role of pSSU1 in S. suis.