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Identification of proteins that interact with NF-YA
K Yamada1, H Osawa, D K Granner
1Department of Biochemistry, Fukui Medical University, Japan. kazuya@fmsrsa.fukui-med.ac.jp
FEBS Letters
|November 26, 1999
Abstract:
We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHXI) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.