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Synthesis and fluorescence properties of Oregon Green 514 labeled peptides
1Centre de Biophysique Moléculaire, UPR 4301 - CNRS affiliée à I'université, Orléans, France.
Abstract:
Oregon Green 514, with high photostability and a pKa of 4.8, is suitable for imaging applications. Labeling of peptide-bound resin with the carboxylic acid form was optimized. Peptide conjugation resulted in altered fluorescence properties of the dye including a quenching of the intensity compared with that in aqueous buffers with a slight red-shift of the emission maximum.
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