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Emesis in the shrew mouse (Suncus murinus) induced by peroral and intraperitoneal administration of staphylococcal
1Department of Veterinary Microbiology, Faculty of Agriculture, Iwate University, Morioka, Japan.
Abstract:
Peroral and intraperitoneal administration of staphylococcal enterotoxin A (SEA) to Suncus murinus elicited an emetic response. The 50% emetic dose of SEA by peroral administration was found to be 32 microg per kg of body weight, whereas that by intraperitoneal administration was 3 microg per kg. Multiple emetic responses occurred 70 to 108 min after peroral administration of an emetic dose of SEA. Similar responses occurred 65 to 102 min after intraperitoneal injection of an emetic dose of SEA. No significant difference in vomiting was observed between male and female animals. Anti-SEA serum neutralized SEA-induced emesis in S. murinus. These findings indicate that S. murinus may serve as a suitable animal model to study the enterotoxigenicity of SEA.
Insights
Staphylococcal enterotoxin A (SEA) causes vomiting in Suncus murinus. This animal model can be used to study SEA enterotoxigenicity and its emetic effects.
Area of Science:
- Microbiology
- Toxicology
- Veterinary Medicine
Background:
- Staphylococcal enterotoxin A (SEA) is a potent toxin responsible for food poisoning.
- Understanding the mechanisms of SEA toxicity and identifying suitable animal models are crucial for research.
Purpose of the Study:
- To investigate the emetic response of Suncus murinus to Staphylococcal enterotoxin A (SEA).
- To evaluate Suncus murinus as a potential animal model for studying SEA enterotoxigenicity.
Main Methods:
- Administration of varying doses of SEA via peroral and intraperitoneal routes to Suncus murinus.
- Observation and recording of emetic responses, including onset and duration.
- Testing the efficacy of anti-SEA serum in neutralizing SEA-induced emesis.
Main Results:
- Both peroral and intraperitoneal administration of SEA elicited a dose-dependent emetic response in Suncus murinus.
- The 50% emetic dose (EID50) was 32 µg/kg (peroral) and 3 µg/kg (intraperitoneal).
- Anti-SEA serum successfully neutralized SEA-induced emesis, confirming SEA's role.
Conclusions:
- Suncus murinus exhibits a clear emetic response to SEA, supporting its use as an animal model.
- This model can facilitate further research into SEA's enterotoxigenicity and the mechanisms of emesis.