Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Selective decrease of mRNAs encoding plasma membrane calcium pump isoforms 2 and 3 in rat kidney.

A J Caride1, E N Chini, J T Penniston

  • 1Department of Biochemistry, Division of Nephrology, Mayo Medical School, Rochester, Minnesota, USA.

Kidney International
|November 26, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Surface color spectrophotometry in a murine model of steatosis: an accurate technique with potential applicability in liver procurement.

Laboratory investigation; a journal of technical methods and pathology·2021
Same author

Plasma-membrane Ca(2+) pumps: structural diversity as the basis for functional versatility.

Biochemical Society transactions·2007
Same author

Modulation of store-operated Ca2+ entry by cyclic-ADP-ribose.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2006
Same author

Reincorporated plasma membrane Ca2+-ATPase can mediate B-Type Ca2+ channels observed in native membrane of human red blood cells.

The Journal of membrane biology·2002
Same author

Expression and cellular distribution pattern of plasma membrane calcium pump isoforms in rat pancreatic islets.

The Journal of membrane biology·2002
Same author

Characterization of the deafwaddler mutant of the rat plasma membrane calcium-ATPase 2.

Hearing research·2001
Same journal

Why current evidence does not support routine C5 inhibition in STEC-HUS.

Kidney international·2026
Same journal

Beyond galactose deficiency: redefining pathogenic IgA in IgA nephropathy.

Kidney international·2026
Same journal

KDIGO Life Cycle of Guideline Development Series Part 6: Bridging the Gap between Guidelines and Clinical Practice: The KDIGO Approach to Global Implementation and Education in Nephrology.

Kidney international·2026
Same journal

KDIGO Life Cycle of Guideline Development Series Part 5: Guideline updates and a living model for the future.

Kidney international·2026
Same journal

Crystal-storing histiocytosis causing severe acute kidney injury.

Kidney international·2026
Same journal

Peritoneal dialysis in a patient with extensive burn scarring.

Kidney international·2026
See all related articles

Reduced expression of plasma membrane calcium ATPase (PMCA) isoforms 2b and 3 in the kidney is linked to hypercalciuria, suggesting their role in calcium reabsorption regulation.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Biochemistry

Background:

  • Multiple isoforms of plasma membrane calcium ATPase (PMCA) exist, but their specific biological functions remain largely unknown.
  • Previous studies identified PMCA isoforms in renal cortical tissue, prompting investigation into their role in kidney function.

Purpose of the Study:

  • To investigate the potential role of PMCA isoforms in tubular reabsorption of calcium (Ca2+).
  • To determine if PMCA isoform expression in the kidney is modulated by pathophysiologic stimuli like hypercalciuria.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to quantify mRNA levels of PMCA1-4 isoforms.
  • Gene expression was analyzed in the renal cortex, liver, and brain of rats with diet-induced hypercalciuria (low-phosphate diet) and controls (high-phosphate diet).

Related Experiment Videos

Main Results:

  • In hypercalciuric rats, mRNAs for PMCA2b and PMCA3(a + c) were significantly reduced (~50%) in the renal cortex compared to controls.
  • No significant changes were observed in PMCA1b and PMCA4 mRNA levels in the renal cortex.
  • Calbindin 28 kDa mRNA levels increased in hypercalciuric rats, while PMCA2b and PMCA3(a + c) mRNA levels remained unchanged in the liver and brain, respectively.

Conclusions:

  • Kidney PMCA isoform expression is selectively modulated by pathophysiologic conditions such as hypercalciuria.
  • The observed decrease in PMCA2b and PMCA3(a + c) mRNA in hypercalciuria suggests these isoforms play a role in tubular calcium reabsorption and its regulation.