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Related Experiment Videos

ACGT-containing abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent.

T Hobo1, M Asada, Y Kowyama

  • 1Center for Molecular Biology and Genetics, Mie University, Kamihama-cho, Japan.

The Plant Journal : for Cell and Molecular Biology
|November 26, 1999
PubMed
Summary

ABA response elements (ABREs) and coupling element 3 (CE3) are functionally equivalent. Both are required for ABA induction and VP1 activation, binding the TRAB1 factor, suggesting they belong to a single class of cis-acting elements.

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Area of Science:

  • Plant molecular biology
  • Gene regulation
  • Abscisic acid signaling

Background:

  • Abscisic acid response elements (ABREs) are crucial for gene regulation.
  • Single ABREs often require coupling elements for full ABA induction.
  • The Osem gene promoter contains both an ACGT-containing ABRE (motif A) and a CE3 sequence.

Purpose of the Study:

  • To investigate the functional relationship between motif A and CE3 in the Osem promoter.
  • To determine the role of these elements in ABA responsiveness and VP1 activation.
  • To clarify the classification of ABREs and related cis-acting elements.

Main Methods:

  • Linker-scan analyses of the Osem promoter fragment.
  • Motif-exchange experiments to assess functional interchangeability.

Related Experiment Videos

  • Electrophoretic mobility shift assays to study TRAB1 binding.

    • Main Results:

    • Both motif A and CE3 are essential for ABA responsiveness and VP1 activation.
    • Motif A and CE3 sequences are functionally interchangeable.
    • The bZIP factor TRAB1 binds to both motif A and CE3, as well as other non-ACGT ABREs.

    Conclusions:

    • ACGT-containing ABREs and CE3 are functionally equivalent cis-acting elements.
    • TRAB1 recognizes multiple types of ABREs, including CE3.
    • All investigated ABREs, including CE3, can be classified as a single category of cis-acting elements.