Functional interaction of cryptochrome 1 and phytochrome D
The Plant Journal : for Cell and Molecular Biology
|November 26, 1999
Summary
Phytochrome D (phyD) partially compensates for phytochrome B (phyB) loss in Arabidopsis, requiring cryptochrome 1 (cry1) for red light responses. This interaction is crucial for photomorphogenesis and light signaling pathways.
Area of Science:
- Plant Biology
- Photomorphogenesis
- Molecular Genetics
Background:
- Phytochromes and cryptochromes are key photoreceptors regulating plant growth and development.
- Previous studies explored interactions between phytochrome A (phyA), phytochrome B (phyB), and cryptochrome 1 (cry1).
- The specific role of phytochrome D (phyD) in conjunction with other photoreceptors remained less understood.
Purpose of the Study:
- To investigate the signal transduction network involving phytochrome D (phyD) in Arabidopsis thaliana.
- To elucidate the functional interaction between phyD, phytochrome B (phyB), and cryptochrome 1 (cry1) in response to light.
- To determine the contribution of phyD to hypocotyl elongation inhibition.
Main Methods:
- Utilized wild-type and mutant Arabidopsis thaliana lines lacking specific photoreceptors (phyA, phyB, phyD, phyE, cry1, cry2).
- Analyzed hypocotyl elongation inhibition using red light pulses following white light pre-irradiation.
- Assessed the reversibility of light responses by far-red light.
Main Results:
- Phytochrome D (phyD) demonstrated a partial ability to substitute for phytochrome B (phyB) function.
- In phyB mutants, red light responses were dependent on the presence of both cry1 and phyD.
- The observed light responses were reversible by far-red light, with a loss of reversibility occurring over time.
Conclusions:
- Phytochrome D (phyD) plays a significant role in mediating light responses, particularly in the absence of functional phytochrome B (phyB).
- The interaction between phyD and cry1 is essential for specific light signaling pathways in Arabidopsis.
- Phytochrome E (phyE) did not show a detectable functional interaction with cry1 in this study.
More Related Videos
14:13Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping
Published on: October 24, 2014
07:55Luciferase Complementation Imaging Assay in Nicotiana benthamiana Leaves for Transiently Determining Protein-protein Interaction Dynamics
Published on: November 20, 2017
Related Concept Videos
02:00Photoreceptors and Plant Responses to Light
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
02:45Biological Clocks and Seasonal Responses
The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.
01:15The Antenna Complex
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency can...
02:19Circadian Rhythms and Gene Regulation
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
01:11Channel Rhodopsins
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...