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Thermodynamics of single mismatches in RNA duplexes
R Kierzek1, M E Burkard, D H Turner
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Noskowskiego.
Biochemistry
|November 26, 1999
Abstract:
The thermodynamic properties and structures of single mismatches in short RNA duplexes were studied in optical melting and imino proton NMR experiments. The free energy increments at 37 degrees C measured for non-GU single mismatches range from -2.6 to 1.7 kcal/mol. These increments depend on the identity of the mismatch, adjacent base pairs, and the position in the helix. UU and AA mismatches are more stable close to a helix end, but GG mismatch stability is essentially unaffected by the position in the helix. Approximations are suggested for predicting stabilities of single mismatches in short RNA duplexes.