Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Single molecule analysis of DNA replication.

J Herrick1, A Bensimon

  • 1Laboratoire de Biophysique de l'ADN, Département des Biotechnologies, Institut Pasteur, 25, rue du Dr.-Roux, 75724 Paris Cedex 15, France.

Biochimie
|November 26, 1999
PubMed
Summary

Molecular combing offers a novel method for genome-wide DNA replication studies. This technique enables precise mapping of replication origins and units, advancing genomic research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correlation between integration of high-risk HPV genome into human DNA detected by molecular combing and the severity of cervical lesions: first results of the EXPL-HPV-002 study.

Ceska gynekologie·2019
Same author

4D Visualization of replication foci in mammalian cells corresponding to individual replicons.

Nature communications·2016
Same author

Sapling biomass allocation and growth in the understory of a deciduous hardwood forest.

American journal of botany·2011
Same author

Molecular combing.

Current protocols in cytometry·2008
Same author

[Cochlear implants in children with mental disabilities].

Revue de laryngologie - otologie - rhinologie·2008
Same author

Genetic variation in IGF1, IGFBP3, IRS1, IRS2 and risk of breast cancer in women living in Southwestern United States.

Breast cancer research and treatment·2006

Area of Science:

  • Genomics
  • Molecular Biology
  • Biophysics

Background:

  • Understanding DNA replication is crucial for cell biology and disease research.
  • Current methods for analyzing replication origins and units have limitations in resolution and scale.

Purpose of the Study:

  • To introduce and validate molecular combing as a novel technique for high-resolution, genome-wide analysis of DNA replication.
  • To demonstrate the utility of molecular combing for studying replication units and origins.

Main Methods:

  • Molecular combing involves stretching genomic DNA molecules on a glass surface using a receding air/water interface.
  • Fluorescence hybridization is applied to combed DNA for high-resolution optical mapping (1-4 kb).
  • Direct labeling of replicating DNA sequences allows for visualization and mapping of replication origins.

Main Results:

  • Molecular combing provides a simple, controlled, and reproducible method for DNA optical mapping.
  • The technique allows for statistically significant measurements by analyzing hundreds of haploid genomes.
  • Replication origins can be directly visualized and mapped with high resolution.

Conclusions:

  • Molecular combing is a powerful and attractive tool for genomic studies of DNA replication.
  • This approach facilitates the analysis of spatial and temporal organization of replication units and origins.
  • The method holds promise for advancing research in DNA replication and genome stability.

Related Experiment Videos