Prebiotic formation of ADP and ATP from AMP, calcium phosphates and cyanate in aqueous solution

Adenosine-5'-triphosphate was synthesized by the phosphorylation of adenosine-5'-diphosphate in aqueous solution containing cyanate as a condensing reagent and insoluble calcium phosphate produced from phosphate and calcium chloride. In a similar manner, adenosine-5'-diphosphate was synthesized from adenosine-5'-monophosphate. When the experiment was carried out in the conditions of 4 degrees C and pH 5.75, the formation of adenosine-5'-diphosphate and adenosine-5'-triphosphate from adenosine-5'-monophosphate was observed in the yields of 19 and 7%, respectively. The other nucleoside-5'-triphosphates were also produced from their respective diphosphates.