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Interphase FISH for rapid identification of a down syndrome animal model
J Strovel1, J Stamberg, P J Yarowsky
1Division of Human Genetics, University of Maryland Medical System, Baltimore, MD, USA.
Cytogenetics and Cell Genetics
|November 27, 1999
Abstract:
Mouse models of Down syndrome (trisomy 21, DS) have been developed to study the consequences of triplication of regions syntenic to distal human chromosome 21. We report a technique for the identification of segmental trisomy 16 (Ts65Dn) and diploid progeny at any age using interphase fluorescence in situ hybridization (FISH) of uncultured tail fibroblasts. Our technique is faster and less expensive than blood karyotyping.